bio-primer-design-primer-validation

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Validate PCR primers for specificity, dimers, hairpins, and secondary structures using primer3-py thermodynamic calculations. Check self-complementarity, heterodimer formation, and 3' stability. Use when validating primer specificity and properties.

By tools-only schedule Updated 2/14/2026

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Install via CLI

npx skills add https://github.com/tools-only/X-Skills --skill bio-primer-design-primer-validation

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